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Second harmonic imaging of exocytosis at fertilization

Andrew C Millard1, Mark Terasaki, Leslie M Loew

  • 1Department of Cell Biology, University of Connecticut Health Center, Farmington, 06030-1507, USA.

Biophysical Journal
|April 26, 2005
PubMed

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During fertilization, an egg and sperm cell fuse to create a new diploid structure. In humans, the process occurs once the egg has been released from the ovary, and travels into the fallopian tubes. The process requires several key steps: 1) sperm present in the genital tract must locate the egg; 2) once there, sperm need to release enzymes to help them burrow through the protective zona pellucida of the egg; and 3) the membranes of a single sperm cell and egg must fuse, with the sperm...