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Related Experiment Videos

Two new fungal inteins.

Margaret I Butler1, Timothy J D Goodwin, Russell T M Poulter

  • 1Department of Biochemistry, University of Otago, Dunedin, New Zealand. margi.butler@stonebow.otago.ac.nz

Yeast (Chichester, England)
|April 26, 2005
PubMed
Summary

Researchers discovered new nuclear inteins in fungi, expanding the known diversity of eukaryotic inteins beyond the VMA intein. These findings reveal novel inteins in glutamate synthase and chitin synthase genes, offering insights into intein evolution.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Mycology

Background:

  • Inteins are protein segments that excise themselves from precursor proteins.
  • Previously, only the VMA intein in yeast vacuolar ATPase was known to be encoded by a eukaryotic nuclear genome.
  • Recent discoveries have identified PRP8 inteins in various yeast and fungal species.

Purpose of the Study:

  • To identify and characterize novel nuclear-encoded inteins in ascomycete fungi.
  • To investigate the phylogenetic relationships of newly discovered inteins with known ones.
  • To explore the evolutionary context of eukaryotic nuclear inteins.

Main Methods:

  • Gene sequencing and analysis of ascomycete species.
  • Identification of inteins within specific genes (GLT1, CHS2).

Related Experiment Videos

  • Phylogenetic analysis of intein sequences.

    • Main Results:

    • Discovery of two new non-allelic inteins: one in the glutamate synthase (GLT1) gene of Debaryomyces hansenii, Candida guilliermondii, and Podospora anserina, and another in the chitin synthase (CHS2) gene of Podospora anserina.
    • GLT1 inteins contain homing endonuclease (HEG) domains and occupy the same site within the glutamate synthase.
    • CHS2 intein also possesses a HEG domain.
    • Phylogenetic analysis revealed relationships among VMA, PRP8, GLT1, and CHS2 inteins, placing them within a broader evolutionary context.

    Conclusions:

    • The discovery of GLT1 and CHS2 inteins significantly expands the known repertoire of nuclear-encoded inteins in eukaryotes.
    • The presence of HEG domains suggests potential roles in intein mobility or regulation.
    • The phylogenetic analysis provides insights into the evolutionary history and diversification of nuclear inteins across different fungal lineages and their relationship to other intein types.