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Updated: Aug 18, 2026

Sequencing of Bacterial Microflora in Peripheral Blood: our Experience with HIV-infected Patients
Published on: June 11, 2011
[Bacterial etiology of acute respiratory infections in HIV/AIDS patients]
Miriam Fina Pérez Monrás1, Nereyda Cabrera Cantelar, María del Carmen Batlle Almodóvar
1Instituto de Medicina Tropical "Pedro Kouri", Ciudad de La Habana, Cuba. miriamp@ipk.sld.cu
Abstract:
A total of 63 HIV/AIDS patients with acute respiratory infections admitted to "Pedro Kouri" Tropical Medicine Institute's hospital from January to August 1997 were studied. 545 samples in all were processed: 153 sputa, 150 hemocultures, 110 pharyngeal exudates, 61 nasal exudates and 63 otic exudates. The correlation of the results from the acridine orange technique, Gram staining and culture was studied. The most frequent bacterial agents causing acute respiratory infections in the group of isolated strains were: Pseudomonas aeruginosa (29.65 %), Staphylococcus aureus (27.14%) and Streptococcus penumoniae (9.04%). The microorganisms more frequently associated with bacteriemias were: Staphylococcus aureus (16.13%), Pseudomonas aeruginosa (12.90%) and Type b Salmonella (12.90%). The acridine orange technique was the most effective in presumptive diagnosis of bacteriemia with 95 % certainty since it showed a positivity range from 40, 56 to 50, 56, with a p < 0,05 which is higher than that of the conventional culture and the Gram staining.
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