Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Neuropeptide Y expression, localization and cellular transducing effects in HUVEC.

Antonio P Silva1, Jocelyne E Kaufmann, Cécile Vivancos

  • 1Division d'Hypertension et de Médecine Vasculaire, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.

Biology of the Cell
|April 27, 2005
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clock gene signature predicts insomnia and links to sleep/circadian parameters.

Translational psychiatry·2026
Same author

Ketogenic diet as a therapeutic strategy for neurodegenerative diseases: from mechanisms to translational challenges.

Translational neurodegeneration·2026
Same author

Tomatidine is a senotherapeutic compound that improves cognitive function and reduces cellular senescence in aged mice.

EMBO molecular medicine·2026
Same author

Systematic review of changes in extracellular vesicles associated with obstructive sleep apnoea: implications for diagnosis and treatment.

European respiratory review : an official journal of the European Respiratory Society·2025
Same author

Regulation of Metabolic Rhythms by Glial Clocks.

Journal of biological rhythms·2025
Same author

Neuroendocrine timekeepers: changes in normal and premature aging.

Trends in molecular medicine·2025

Human umbilical vein endothelial cells (HUVEC) produce, store, and respond to neuropeptide Y (NPY). This suggests NPY plays an autocrine role in regulating endothelial cells.

Area of Science:

  • Endothelial Cell Biology
  • Neuroendocrinology

Background:

  • Neuropeptide Y (NPY) is known to influence vascular endothelial cell properties, including angiogenesis and vasoconstriction.
  • While NPY has been detected in human umbilical vein endothelial cells (HUVEC), its synthesis, storage, and secretion mechanisms were uncharacterized.

Purpose of the Study:

  • To investigate the expression, storage, and cellular signaling effects of NPY in HUVEC.

Main Methods:

  • ELISA for NPY and pro-NPY quantification.
  • RT-PCR for gene expression analysis.
  • Immunofluorescence and electron microscopy for subcellular localization.
  • Intracellular calcium measurements to assess receptor function.

Main Results:

Related Experiment Videos

  • HUVEC express and store NPY in distinct vesicular compartments, separate from von Willebrand factor.
  • NPY and Rab3A showed partial co-localization in granular structures.
  • NPY immunoreactivity was observed in vesicles, mitochondria, and the nucleus.
  • Functional Y1 and Y2 receptors were present, mediating intracellular calcium increases upon NPY stimulation.
  • NPY did not induce acute von Willebrand factor secretion.

    • Conclusions:

    • HUVEC synthesize, store, and respond to NPY.
    • These findings suggest an autocrine regulatory role for NPY within the endothelium.