Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The correlation between sampling site and gene expression in the term human placenta.

S M Wyatt1, F T Kraus, C-R Roh

  • 1Department of OBGYN, Washington University School of Medicine, St Louis, MO 63110, USA.

Placenta
|April 27, 2005
PubMed
Summary

Hypoxia-related gene expression in human placenta varies by sampling location. Specific genes like VEGF and CTGF were higher in the lateral border, correlating with placental maturation and perfusion differences.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Association of intraplacental oxygenation patterns on dual-contrast MRI with placental abnormality and fetal brain oxygenation.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2022
Same author

Structure dependence and species sensitivity of in vivo hepatobiliary toxicity with lysophosphatidic acid receptor 1 (LPA<sub>1</sub>) antagonists.

Toxicology and applied pharmacology·2022
Same author

Outcomes of pregnancy and delivery in women with cardiac disease compared to matched healthy controls.

The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·2021
Same author

Hydroxychloroquine treatment during pregnancy in lupus patients is associated with lower risk of preeclampsia.

Lupus·2019
Same author

History-indicated cervical cerclage in management of twin pregnancy.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2018
Same author

Centennial-scale reductions in nitrogen availability in temperate forests of the United States.

Scientific reports·2017

Area of Science:

  • Reproductive biology
  • Placental development
  • Molecular genetics

Background:

  • Hypoxia influences placental gene expression.
  • Previous studies identified hypoxia-up-regulated transcripts in trophoblasts.
  • The spatial distribution of these transcripts within the placenta is not well understood.

Purpose of the Study:

  • To investigate if hypoxia-related placental transcript expression is site-dependent within the term human placenta.
  • To correlate gene expression patterns with villous histology and placental site.
  • To determine the influence of placental sampling location on gene expression.

Main Methods:

  • Analysis of nine distinct sites from six term human placentas.
  • Quantitative PCR (qPCR) to determine relative gene expression levels.

Related Experiment Videos

  • Correlation of gene expression with villous histology and placental sampling site.

    • Main Results:

    • Vascular Endothelial Growth Factor (VEGF) and Connective Tissue Growth Factor (CTGF) expression were significantly higher (1.6-2.9 fold) in the subchorionic lateral border compared to medial basal sites.
    • Expression of cytoskeleton proteins (lamininA3, alpha-tubulin) and signal transduction protein Rad also increased in the lateral border.
    • Enhanced villous maturation, syncytial knots, and fibrin deposits were observed in the lateral border and correlated with increased hypoxia-related transcript expression.
    • NDRG1, adipophilin, and human placental lactogen expression remained unchanged across sites.
    • No site-dependent expression differences were found in placentas with marginal cord insertion.

    Conclusions:

    • Hypoxia-related gene expression in the term human placenta is significantly dependent on the sampling site within the placental disk.
    • Observed spatial variations in gene expression likely reflect local differences in villous perfusion and placental maturation.
    • Findings highlight the importance of standardized sampling protocols in placental research.