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Updated: Aug 18, 2026

Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro
Published on: June 28, 2024
[The determination of glutathione by electrochemiluminescence quenching method]
Ying-ying Qi1, Ying Wu, Jun-wei Di
1College of Chemistry and Chemical Engineering, Suzhou University, Suzhou 215006, China.
Abstract:
Glutathione is an important active substance in organisms. Its study and determination are very significant to human being's health and life. In neutral medium, the quenching effect of reduced glutathione on the electrochemiluminescence of luminol-iodide was studied in this paper. Therefore a novel sensitive method for the determination of reduced glutathione was developed based on it. The linear response range of this method is from 3.38 x 10(-13) mol x L(-1) to 4.72 x 10(-3) mol x L(-1). This method is the most sensitive one and its linear range is the widest among those reported in the literature.

