Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Inflammatory gene signature in ulcerative colitis with cDNA macroarray analysis.

S Okahara1, Y Arimura, T Yabana

  • 1First Department of Internal Medicine, Sapporo Medical University, Sapporo, Japan. okahara@sapmed.ac.jp

Alimentary Pharmacology & Therapeutics
|April 28, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transcriptome characterization of male accessory glands in ants to identify molecules involved in their reproductive success.

Insect molecular biology·2017
Same author

Adherence to clinical practice guidelines for the management of Clostridium difficile infection in Japan: a multicenter retrospective study.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2017
Same author

Estimation of the congestion area volume in potential living donor remnant livers.

Transplantation proceedings·2013
Same author

Real-time monitoring of liver damage during experimental ischaemia-reperfusion using a nitric oxide sensor.

The British journal of surgery·2012
Same author

Consumption of oxygen: a mitochondrial-generated progression signal of advanced cancer.

Cell death & disease·2012
Same author

Clinical-evaluation of serum basic fetoprotein as a marker in urogenital malignant-tumors.

Oncology reports·2011
Same journal

Letter: HBsAg Loss After COVID-19 Vaccination: Implications for HBV Screening, Immunity Monitoring, and Health Worker Protection in LMICs. Authors' Reply.

Alimentary pharmacology & therapeutics·2026
Same journal

Editorial: Mind the Gap-Peripheral Immune Signatures of HBV Cirrhosis in an Underserved Population.

Alimentary pharmacology & therapeutics·2026
Same journal

Editorial: Fatigue in Disorders of Gut-Brain Interaction-An Important Symptom to Recognise.

Alimentary pharmacology & therapeutics·2026
Same journal

Letter: Non-Invasive Tests After Semaglutide in MASH as Disease-Activity Readouts Rather Than Response Biomarkers.

Alimentary pharmacology & therapeutics·2026
Same journal

Editorial: Non-Selective Beta-Blockers in Decompensated Cirrhosis-More Friends Than Foes.

Alimentary pharmacology & therapeutics·2026
Same journal

Letter: Reconciling Histological Fibrosis Improvement With Magnetic Resonance Elastography After Sleeve Gastrectomy.

Alimentary pharmacology & therapeutics·2026
See all related articles

Gene expression changes in ulcerative colitis (UC) inflamed colon mucosa were identified using cDNA macroarray. This study highlights key proinflammatory and anti-inflammatory genes, offering potential diagnostic and therapeutic targets for active UC.

Area of Science:

  • Gastroenterology
  • Molecular Biology
  • Genomics

Background:

  • Previous ulcerative colitis (UC) research primarily focused on identifying susceptibility genes.
  • Understanding gene expression dynamics during UC inflammation is crucial for advancing treatment.

Purpose of the Study:

  • To investigate gene expression alterations in colon mucosa during active ulcerative colitis (UC) inflammation.
  • To identify novel diagnostic markers and therapeutic targets for UC.

Main Methods:

  • Utilized cDNA macroarray to analyze gene expression profiles of approximately 1300 genes.
  • Collected colonoscopic biopsy specimens from 23 ulcerative colitis patients (inflamed and non-inflamed).
  • Confirmed significant gene expression changes using real-time polymerase chain reaction.

Related Experiment Videos

Main Results:

  • Identified five upregulated genes in inflamed mucosa: proinflammatory (MRP14, GRO gamma, SAA1) and anti-inflammatory (TIMP1, Elafin).
  • Detected one upregulated gene (L-FABP) in non-inflamed mucosa.
  • Macroarray analysis accurately reflected gene expression in the clinical UC setting.

Conclusions:

  • The identified genes are directly applicable to diagnostics for active ulcerative colitis.
  • These genes represent potential novel therapeutic targets for ulcerative colitis treatment.