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Published on: December 1, 2011
[Sequence analysis of 3'-non-coding region of RHD]
1Sequence Shenzhen Blood Center, Shenzhen 518035, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi
|April 28, 2005
Summary
The 3' non-coding region of the RHD gene is identical in Rh-positive and D(el) red blood cells. This suggests that the D antigen
Area of Science:
- Genetics
- Molecular Biology
- Immunology
Context:
- The Rhesus (Rh) blood group system is crucial in transfusion medicine and diagnostics.
- The D antigen, a key component of the Rh system, exhibits variable expression, notably in D(el) red blood cells.
- The 3' non-coding region of the RHD gene, located after the stop codon, plays a role in gene regulation.
Purpose:
- To determine the DNA sequence of the 3' non-coding region of the RHD gene.
- To investigate potential genetic variations in the 3' non-coding region associated with D(el) red blood cells.
Summary:
- Polymerase chain reaction (PCR) and direct sequencing were used to analyze the 3' non-coding region of the RHD gene in Rh-positive and D(el) samples.
- The study found identical sequences in the 3' non-coding region (103 bp between the RHD coding region's 3'-end and the Rhesus box's 5'-end) across all tested samples.
- D(el) samples exhibited the same sequence as normal Rh-positive samples, indicating no sequence variation in this region.
Impact:
- The findings suggest that the lower expression of the D antigen in D(el) red blood cells is not attributable to alterations in the 3' non-coding region of the D(el) gene.
- This research contributes to understanding the molecular basis of RhD antigen expression variability.
- Provides a foundation for further investigation into other regulatory elements or factors influencing D antigen expression.
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