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Lipid peroxidation in stored red cells.

J A Knight1, R P Voorhees, L Martin

  • 1Laboratory Service, Veterans Administration Medical Center, Salt Lake City, Utah.

Transfusion
|May 1, 1992
PubMed
Summary

Metal chelators like deferoxamine mesylate significantly reduce lipid peroxidation in stored red blood cells. This finding suggests potential strategies to improve the viability and longevity of transfused red cells.

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Area of Science:

  • Biochemistry
  • Hematology
  • Oxidative Stress Research

Background:

  • Lipid peroxidation, initiated by oxygen-free radicals, is elevated in various diseases and conditions affecting red blood cells.
  • Reactive iron species and heme moieties contribute to increased lipid peroxidation in red cells.
  • Understanding and mitigating lipid peroxidation is crucial for red blood cell preservation.

Purpose of the Study:

  • To investigate the effects of various compounds on lipid peroxidation in stored red blood cells.
  • To evaluate the efficacy of metal chelators and antioxidants in reducing malondialdehyde production.
  • To determine potential methods for enhancing the viability of transfused red cells.

Main Methods:

  • Stored blood samples were treated with deferoxamine mesylate, diethylenetriaminepentaacetic acid, glutathione, dimercaptosuccinic acid, and ascorbic acid.
  • Malondialdehyde concentrations, an indicator of lipid peroxidation, were measured.
  • Statistical analysis (p-values) was used to determine the significance of the observed changes.

Main Results:

  • Deferoxamine mesylate and diethylenetriaminepentaacetic acid significantly reduced malondialdehyde levels (p < 0.001).
  • Glutathione showed less effectiveness as an antioxidant (p < 0.01 or p < 0.05).
  • Dimercaptosuccinic acid and ascorbic acid increased malondialdehyde production (p < 0.001 and p < 0.05, respectively). Ascorbic acid did not enhance deferoxamine mesylate's protective effect.

Conclusions:

  • Metal chelators, particularly deferoxamine mesylate and diethylenetriaminepentaacetic acid, are effective in reducing lipid peroxidation in stored red blood cells.
  • Certain compounds, like dimercaptosuccinic acid and ascorbic acid, may exacerbate lipid peroxidation.
  • The addition of specific metal chelators to stored blood shows promise for improving the viability and longevity of transfused red cells.

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