Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Molecular recognition by a binary code.

Frederic A Fellouse1, Bing Li, Deanne M Compaan

  • 1Department of Protein Engineering, Genentech Inc, 1 DNA Way, South San Francisco, CA 94080, USA.

Journal of Molecular Biology
|April 28, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Greasing the wheels of inflammasome formation: regulation of NLRP3 function by S-linked fatty acids.

Biochemical Society transactions·2025
Same author

S-acylation of NLRP3 provides a nigericin sensitive gating mechanism that controls access to the Golgi.

eLife·2024
Same author

A sensitive cell-based assay for testing potency of botulinum neurotoxin type A

ALTEX·2024
Same author

Tetherin antagonism by SARS-CoV-2 ORF3a and spike protein enhances virus release.

EMBO reports·2023
Same author

Establishing SARS-CoV-2 membrane protein-specific antibodies as a valuable serological target via high-content microscopy.

iScience·2023
Same author

Sec22b is a critical and nonredundant regulator of plasma cell maintenance.

Proceedings of the National Academy of Sciences of the United States of America·2023

Researchers created functional antibodies using a limited set of amino acids, tyrosine and serine. These minimalist antibodies demonstrated high affinity and specificity, revealing the fundamental principles of molecular recognition.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Background:

  • Antibodies are crucial for molecular recognition in biological systems.
  • The diversity of amino acids in natural antibodies contributes to their recognition capabilities.
  • Exploring minimalist antibody designs can elucidate fundamental principles of molecular recognition.

Purpose of the Study:

  • To investigate the feasibility of generating functional antibodies from a restricted amino acid library.
  • To determine the binding affinity and specificity of antibodies derived from a binary code of tyrosine and serine.
  • To elucidate the structural basis of molecular recognition in minimalist antibody-antigen complexes.

Main Methods:

  • Construction of an antibody library using a binary code restricted to tyrosine and serine.

Related Experiment Videos

  • Generation and characterization of antibodies against human vascular endothelial growth factor (VEGF).
  • Affinity and specificity assessment using cell-based assays.
  • X-ray crystallography of an antigen-binding fragment complexed with human death receptor 5 (DR5).

    • Main Results:

    • Functional antibodies were successfully generated from a library restricted to tyrosine and serine.
    • An antibody against human VEGF exhibited high affinity (K(D)=60 nM) and specificity.
    • Structural analysis revealed the molecular recognition mechanisms of a minimalist antibody-antigen complex.
    • Tyrosine and serine residues were identified as intrinsically suitable for molecular recognition.

    Conclusions:

    • Molecular recognition can be achieved with a highly restricted set of amino acids.
    • Minimalist antibody designs provide insights into the fundamental principles of molecular recognition.
    • The study demonstrates that molecular recognition can evolve from simple chemical diversity.