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Do preservation solutions protect rat cremaster microcirculation during ischemia and reperfusion?
Jacqueline Bastiaanse1, Dick W Slaaf, Mirjam G A oude Egbrink
1Department of Plastic, Reconstructive & Hand Surgery, Utrecht University Medical Center, Utrecht, the Netherlands. jbas@spch.azm.nl
The Journal of Surgical Research
|April 28, 2005
Summary
Celsior solution worsened microcirculation in rat cremaster muscle during ischemia and reperfusion, unlike HTK solution. HTK is better for preserving muscle tissue.
Area of Science:
- Physiology
- Biomedical Engineering
- Organ Preservation
Background:
- Investigating preservation solutions for microcirculation during ischemia-reperfusion is critical.
- Celsior solution was hypothesized to be more protective than HTK due to its antioxidant content.
Purpose of the Study:
- To evaluate Celsior solution's efficacy in protecting rat cremaster muscle microcirculation.
- To compare Celsior's protective effects against HTK (histidine-tryptophan-ketoglutarate-Bretschneider solution).
Main Methods:
- Intravital microscopy examined capillary perfusion and leukocyte-endothelium interactions in rat cremaster muscle.
- Muscle tissue was perfused with Celsior or HTK, followed by 4-6 hours of warm ischemia and 2 hours of reperfusion.
Main Results:
- Celsior significantly deteriorated capillary perfusion after 4 and 6 hours of ischemia.
- HTK did not significantly influence capillary perfusion.
- Both solutions similarly reduced leukocyte-endothelium interactions.
Conclusions:
- Preischemic Celsior perfusion adversely affected capillary perfusion in rat cremaster muscle.
- HTK demonstrated a neutral effect on capillary perfusion.
- HTK is more suitable for muscular tissue preservation than Celsior.