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Related Experiment Videos

IgG and complement receptor expression in children treated by peritoneal dialysis.

Antonia H M Bouts1, Jean-Claude Davin, Raymond T Krediet

  • 1Emma Children's Hospital AMC, University of Amsterdam, The Netherlands. a.h.bouts@amc.uva.nl

Pediatric Nephrology (Berlin, Germany)
|April 28, 2005
PubMed
Summary

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Children on peritoneal dialysis (PD) face higher infection risks. Their white blood cells show altered IgG receptor (FcgammaR) and complement receptor (CR) expression, indicating potential immune activation during peritonitis.

Area of Science:

  • Immunology
  • Nephrology
  • Pediatrics

Background:

  • Children undergoing peritoneal dialysis (PD) exhibit an elevated susceptibility to infections.
  • White blood cells (WBCs) utilize IgG receptors (FcgammaRs) and complement receptors (CRs) for phagocytosis, a key immune defense mechanism.

Purpose of the Study:

  • To investigate the expression levels of FcgammaRs and CRs on monocytes, macrophages, and neutrophils in both blood and peritoneal dialysis effluent (PDE) of children on PD.
  • To assess how FcgammaR and CR expression changes in response to PD treatment and peritonitis episodes.

Main Methods:

  • Blood and PDE samples from 39 PD children were analyzed using flow cytometry.
  • Cells were labeled with monoclonal antibodies targeting specific FcgammaRs (CD16, CD32, CD64) and CRs (CD11b, CD35).

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  • Expression was quantified by measuring the percentage of positive cells and mean fluorescence intensity (MFI).

    • Main Results:

    • Peritoneal cells displayed lower percentages of FcgammaR- and CR-positive cells but higher MFI (receptor number per cell) compared to blood cells, suggesting activation.
    • FcgammaR and CR expression in blood and dialysate remained stable during the first year of PD.
    • During peritonitis, blood monocyte receptor MFI increased (except CD32), while PDE macrophage and neutrophil percentages rose, though MFI changes were inconsistent.

    Conclusions:

    • Peritoneal cells in PD children show signs of activation with reduced receptor-positive cell counts but increased receptor density.
    • Both blood and peritoneal cells can up-regulate receptor expression during peritonitis, but this response may not reach its maximum capacity.