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Gene expression profiling in hypothalamus of immobilization-stressed mouse using cDNA microarray.

Han-Chang Lee¹, Dong-Eun Chang, Mijung Yeom

¹Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University, Kihung-up, Yongin-si, Kyungki-do 449-701, Korea.

Brain Research. Molecular Brain Research

|April 29, 2005

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Repeated immobilization stress significantly alters gene expression in mouse hypothalamus, affecting metabolism and apoptosis pathways. This study identifies novel genes potentially useful for treating chronic stress and depression.

Area of Science:

Neuroscience
Molecular Biology
Genomics

Background:

Chronic stress impacts the hypothalamic-pituitary-adrenal (HPA) axis.
Understanding stress-induced genomic changes is crucial for developing therapeutics.

Purpose of the Study:

To investigate the effects of repeated immobilization stress on the hypothalamic transcriptome.
To identify novel genes modulated by chronic stress.

Main Methods:

cDNA microarray analysis of hypothalamic tissue from stressed mice.
Quantitative RT-PCR validation of selected gene expression changes.
Bioinformatic analysis of transcriptome data.

Main Results:

108 out of 6016 genes showed significant modulation in the hypothalamus.

Related Experiment Videos

Genes related to energy metabolism, lipid metabolism, apoptosis, and signal transduction were upregulated.

Genes involved in DNA repair, protein biosynthesis, and structure integrity were downregulated.

Dozens of novel genes related to apoptosis, tumor suppression, DNA-binding, and protein folding were identified.

Conclusions:

Repeated immobilization stress induces widespread genomic alterations in the hypothalamus.
Identified novel genes represent potential therapeutic targets for chronic stress and depression.