Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Application of NIR spectroscopy in polymorphic analysis: study of pseudo-polymorphs stability.

Marcelo Blanco1, Dámarih Valdés, Isidro Llorente

  • 1Unidad de Química Analítica, Departamento de Química, Facultad de Ciencias, Universidad Autónoma de Barcelona, Edificio Cn, 08193 Bellaterra, Barcelona, Spain. marcel.blanco@uab.es

Journal of Pharmaceutical Sciences
|April 29, 2005
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Consensus about Health constance for children and adolescents in order to develop phisical activities and sports. Update 2021].

Archivos argentinos de pediatria·2021
Same author

A Novel Domino Reaction: Cyclization of Alkynyl Sulfides by Reaction with IPy<sub>2</sub> BF<sub>4</sub>.

Angewandte Chemie (International ed. in English)·2018
Same author

Determination of drug, excipients and coating distribution in pharmaceutical tablets using NIR-CI.

Journal of pharmaceutical analysis·2018
Same author

Calibration sets selection strategy for the construction of robust PLS models for prediction of biodiesel/diesel blends physico-chemical properties using NIR spectroscopy.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2017
Same author

Pesticide exposure and health conditions of terrestrial pesticide applicators in Córdoba Province, Argentina.

Cadernos de saude publica·2015
Same author

Analysis of ecstasy in oral fluid by ion mobility spectrometry and infrared spectroscopy after liquid-liquid extraction.

Journal of chromatography. A·2015

Near infrared spectroscopy (NIRS) revealed that azithromycin

Area of Science:

  • Pharmaceutical Sciences
  • Solid-State Chemistry

Background:

  • Azithromycin exists in multiple pseudo-polymorphic forms, including anhydrous, monohydrate (MH), and dihydrate (DH).
  • Understanding the stability and transformation of these forms is crucial for drug formulation and efficacy.

Purpose of the Study:

  • To investigate the accelerated transformation of azithromycin pseudo-polymorphs under stressed conditions.
  • To develop and validate a Near Infrared Spectroscopy (NIRS) method for quantifying azithromycin dihydrate (DH) in monohydrate (MH) samples.

Main Methods:

  • Near Infrared Spectroscopy (NIRS) was employed to analyze azithromycin pseudo-polymorphs.
  • Samples were stored at 60°C and 100% relative humidity for 60 days.
  • Partial Least-Squares Regression (PLSR) was used for quantitative analysis.

Related Experiment Videos

Main Results:

  • The amorphous anhydrous form transformed into the dihydrate (DH) form under high temperature and humidity.
  • The monohydrate (MH) and dihydrate (DH) crystalline forms demonstrated stability.
  • The developed NIRS method showed accurate quantification of DH in MH samples, with limits of detection (LD) and quantitation (LQ) comparable to X-ray diffraction (XRD).

Conclusions:

  • NIRS is a reliable technique for monitoring azithromycin pseudo-polymorph transformations.
  • The developed NIRS method is suitable for pharmaceutical industry applications in quality control.
  • Azithromycin crystalline forms exhibit good stability, while the amorphous form is prone to hydration.