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Related Experiment Videos

Microfluidics and chromatography with an atomic force microscope.

Mark S Anderson1

  • 1Jet Propulsion Laboratory, California Institute of Technology, 4800 Oak Grove Drive, M/S 125-109, Pasadena, California 91109, USA. mark.s.anderson@jpl.nasa.gov

Analytical Chemistry
|April 30, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

4.4K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
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This study introduces a novel atomic force microscope (AFM) and Raman spectrometer integrated microfluidic device for efficient fluid pumping, sampling, and trace chemical analysis using shear-driven techniques.

Area of Science:

  • Analytical Chemistry
  • Microfluidics
  • Spectroscopy

Background:

  • Conventional microfluidic pumping methods face limitations in flow rate and precision.
  • Trace chemical analysis in microfluidic systems requires sensitive detection and precise sample handling.

Purpose of the Study:

  • To develop and demonstrate an integrated atomic force microscope (AFM) and Raman spectrometer as a microfluidic device.
  • To utilize AFM for shear-driven pumping, fluidic switching, and sample manipulation.
  • To achieve sensitive trace chemical analysis using surface-enhanced Raman spectroscopy (SERS) localized near the AFM tip.

Main Methods:

  • Integration of an atomic force microscope (AFM) with a Raman spectrometer within a microfluidic chip.
  • Employing the AFM tip-cantilever for shear-driven pumping of fluids in microchannels.

Related Experiment Videos

  • Utilizing AFM's precise fluid translation capabilities for sample injection and switching.
  • Performing surface-enhanced Raman spectroscopy (SERS) with spatial localization provided by the AFM tip.

    • Main Results:

    • Demonstrated shear-driven pumping for rapid fluid flow in microchannels, overcoming conventional pumping limitations.
    • Showcased AFM's capability for sub-femtoliter fluid manipulation, enabling precise sample injection.
    • Successfully integrated AFM imaging of liquid surfaces with SERS for localized chemical analysis of sampled fluids.
    • Achieved sensitive spectral analysis of trace chemicals within the microfluidic device.

    Conclusions:

    • The integrated AFM-Raman microfluidic device is feasible for advanced analytical applications.
    • Shear-driven pumping and AFM-based sample handling offer significant advantages for microfluidic systems.
    • This approach holds potential for developing nanometer-scale chromatography and highly sensitive trace chemical detection.