Nucleotide sequence of canine c-N-ras: codons 1 to 71

The c-N-ras gene has been implicated often in the genesis and/or progression of human leukemias. To our knowledge, the sequence of this gene in the dog has not been reported. Using a system of asymmetric reamplification of double-stranded polymerase chain reaction (PCR) products, we have sequenced normal canine c-N-ras mRNA from position -26 to +213, including codons 12, 13, and 61, which are the sites where oncogenic mutations are most commonly observed. The canine c-N-ras sequence has close homology with the human sequence in this area; there were only 6 observed base differences in nucleotide sequence and none resulted in a change of the encoded amino acid. The results of this study set the stage for directed searches for c-N-ras mutations in experimentally induced and naturally acquired neoplasms of the dog.