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Updated: Aug 15, 2026
Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer
Published on: November 3, 2013
Genetic changes in localized prostate cancer of Japanese patients shown by comparative genomic hybridization
Kotaro Kasahara1, Takahiro Taguchi, Ichiro Yamasaki
1Department of Urology, Kochi Medical School, Nankoku, Kochi, Japan.
Abstract:
To search for additional amplification and deletion sites that may serve as a starting point for the discovery of new oncogenes or tumor suppressor genes, 30 Japanese localized prostate cancers were analyzed by comparative genomic hybridization (CGH) in this study. CGH was used to search for changes in DNA sequence copy-number in a series of 30 primary prostate adenocarcinomas, consisting of 22 cases of pT2N0 (organ confined; without capsular invasion) and 8 cases of pT3N0 (with capsular invasion), removed by radical prostatectomy. CGH revealed that the shortest regions of overlap (SRO) of gains in pT2N0 were at 8q22.2 approximately q24.2, 11q13.1 approximately q14.1, and 12q23 approximately q24.2, whereas the SRO of losses were seen at 8p23.3 approximately p22, 13q21.2 approximately p22, and 18q21 approximately q22. The SRO of gains in pT3N0 were noted at 5q32 approximately q34, 8q22.3 approximately q24.1, 11q14.1 approximately q22.3, and 12q22 approximately q24.2, whereas the SRO of losses were seen at 18q21.2 approximately q23. These results suggest that gains or losses of DNA in these regions are important for prostate cancer progression. The detection of the SRO may serve as a starting point to discover novel oncogenes and tumor suppressor genes involved in prostate cancer progression.
Insights
This study analyzed 30 prostate cancers using comparative genomic hybridization (CGH) to identify DNA copy-number changes. Findings highlight specific genomic regions associated with prostate cancer progression, potentially revealing new oncogenes and tumor suppressor genes.
Area of Science:
- Genetics
- Oncology
- Genomic Medicine
Background:
- Prostate cancer progression is linked to genetic alterations.
- Identifying new oncogenes and tumor suppressor genes is crucial for targeted therapies.
Purpose of the Study:
- To identify novel amplification and deletion sites in localized prostate cancer.
- To discover new oncogenes or tumor suppressor genes involved in prostate cancer progression.
Main Methods:
- Comparative genomic hybridization (CGH) was performed on 30 Japanese localized prostate cancer samples.
- Analysis focused on DNA sequence copy-number changes in primary prostate adenocarcinomas (pT2N0 and pT3N0 stages).
Main Results:
- Specific shortest regions of overlap (SRO) for DNA gains and losses were identified in both pT2N0 and pT3N0 stages.
- Regions with gains included 8q, 11q, and 12q in pT2N0, and 5q, 8q, 11q, and 12q in pT3N0.
- Regions with losses included 8p, 13q, and 18q in pT2N0, and 18q in pT3N0.
Conclusions:
- Genomic alterations in the identified regions are significant for prostate cancer progression.
- These SROs serve as critical starting points for discovering novel cancer-related genes.
