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Related Experiment Videos

An improved mechanical technique for assisted hatching.

Q F Lyu1, L Q Wu, Y P Li

  • 1National Laboratory of Medical Genetics of China, Central South University, Changsha, 410078, People's Republic of China.

Human Reproduction (Oxford, England)
|April 30, 2005
PubMed
Summary
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Assisted hatching (AH) zona opening size is critical for blastocyst hatching. A new long zona dissection (LZD) technique significantly improves hatching rates in mouse and human embryos, overcoming limitations of smaller openings.

Area of Science:

  • Embryology
  • Assisted Reproductive Technology (ART)

Background:

  • Assisted hatching (AH) shows varied clinical outcomes.
  • The adequacy of zona opening size for blastocyst hatching requires investigation.

Purpose of the Study:

  • To evaluate if current assisted hatching (AH) zona opening sizes are sufficient for blastocyst hatching.
  • To develop and assess a new method for improving blastocyst hatching.

Main Methods:

  • A novel technique, long zona dissection (LZD), was developed.
  • Experiments compared LZD and partial zona dissection (PZD) effects on mouse and human embryo hatching in vitro.

Main Results:

  • LZD creates a zona slit exceeding two-thirds of the zona diameter.
  • LZD significantly enhanced mouse blastocyst hatching speed and complete hatching rate (93.9%).

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  • 100% of human blastocysts achieved complete hatching with LZD; smaller openings led to inner cell mass (ICM) entrapment (53.3% vs 12.5%).

    • Conclusions:

    • Moderate zona openings from AH are insufficient for complete in vitro blastocyst hatching.
    • Long zona dissection (LZD) significantly improves blastocyst hatching completion rates.