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Large-scale analysis of human alternative protein isoforms: pattern classification and correlation with subcellular
Mitsuteru Nakao1, Roberto A Barrero, Yuri Mukai
1Human Genome Center, Institute of Medical Science, University of Tokyo Tokyo, Japan.
Nucleic Acids Research
|April 30, 2005
Summary
This study explores human alternative protein isoforms, revealing common patterns like altered C-terminal regions and internal insertions. These variations influence protein function and subcellular localization, enriching the human proteome.
Area of Science:
- Proteomics
- Molecular Biology
- Bioinformatics
Background:
- Alternative protein isoforms arise from complex gene expression.
- Understanding these isoforms is key to comprehending proteome diversity.
Purpose of the Study:
- To classify human alternative protein isoforms.
- To investigate co-occurrence patterns and their relationship with subcellular localization.
- To provide a database of alternative protein isoforms.
Main Methods:
- Analysis of >2600 genes using full-length cDNA clones and SwissProt database.
- Classification and co-occurrence analysis of protein isoforms.
- Prediction of subcellular localization changes.
Main Results:
- Identified C-terminal and internal insertion as the most abundant isoform patterns (43%).
- Observed frequent co-occurrence combinations suggesting structural relationships.
- Predicted terminal changes impact subcellular localization, particularly targeting peroxisomes and mitochondria.
Conclusions:
- Alternative splicing and related events significantly enrich the human proteome.
- Isoform patterns suggest underlying structural relationships.
- Terminal modifications frequently alter protein subcellular targeting.