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[Effect of sulphamethazine on the gene expression of FRTL-5 cells]
Jin-Tao Wang1, Ding Sun, Hong-Mei Pan
1West China School of Public Health, Sichuan University, Chengdu 610041, China.
Objective:
To study the effect of Sulphamethazine on the gene expression of FRTL-5 cells, and to explore the mechaniams of environmental thyroid hormone disruptors.
Methods:
cDNA microarray technique was used to analyze the gene expression of FRTL-5 cells of exponential phase treated by 2.0 microg/ml Sulphamethazine for 24 h. Total RNA from treated and untreated cells were labeled by Cy3 dCTP and Cy5 dCTP respectively. The ratios of Cy3/Cy5 were calculated in order to find the genes which expressed differently.
Results:
There were 679 genes (approximately 7%, total 9753 genes) exhibiting different expression, in which 395 (4.0%) genes up-regulated and 284 (3.0%) genes down-regulated. These genes related to regulation of gene expression, regulation of cell cycle, metabolism in cells, and so on.
Conclusion:
The effect of Sulphamethazine on FRTL-5 cells may be related with a series of genes.
