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[Effects of tea polyphenols and tea pigments on apoptosis in HepG2 cell line]
Xu-Dong Jia1, Chi Han, Jun-Shi Chen
1Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Objective:
To investigate the effect of tea polyphenols and tea pigments on apoptosis in HepG2 cells.
Methods:
HepG2 cells were seeded at a density of 5 x 10(5)/well in six-well culture dishes. The cells were then treated with 50 or 100 mg/L tea pigments and harvested at 48h by trypsinization. Agrose electrophoresis was applied to investigate DNA-LADDER, Bcl-2 and Bax protein expression were detected by Western blot.
Results:
Tea polyphenols and tea pigments induced the appearance of DNA-LADDER; Western blot analysis demonstrated that Bcl-2 expression was significantly inhibited and the expression of Bax was significantly induced by tea polyphenols and tea pigments.
Conclusion:
Induction of apoptosis may be an important mechanism of cancer chemoprevention by tea.
