Related Experiment Video
Updated: Aug 18, 2026
Osmotic Minipump Implantation for Increasing Glucose Concentration in Mouse Cerebrospinal Fluid
Published on: April 7, 2023
[Effects of selenoprotein on blood glucose, Ca2+ transfer and NO system in diabetic mice]
Jun Luo1, Shi-Lian Zhang, Wei Wang
1Hebei Center of Prevent and Control Disease, Shijiazhuang 050021, China.
Objective:
To study the controlling effect of selenoprotein on blood glucose, Ca2+ transfer and NO system in diabetic mice.
Methods:
Male Kunming mice of (20.3 +/- 1.7) g body weight were injected 200 mg/kg bw, 2% alloxan in abdomen to make diabetic model (DM), and were randomly divided into six groups: normal control group (I), normal + selenoprotein group(Se 100 microg/kg bw) (lI), DM control group (III), DM + lower dose selenoprotein group(Se 100 microg/kg bw) (IV), DM + higher dose selenoprotein group (Se 300 microg/kg bw) (V), DM + Na2SeO3 group (Se 100 microg/kg bw) (VI).
Results:
The level of blood glucose in V group [(20.4 +/- 6.3 mmol/L] were significantly lower than lII group(45.3 +/- 3.3) mmol/L P < 0.05. The activity of Ca(2+) -ATPase of kidney in V group (0.90 +/- 0.5 micromol/ (h x mg prot) is significantly higher than III group [(0.35 +/- 0.1) micromol/(h x mg prot)] (P < 0.05, and the activity of NOS in V group [(25.0 +/- 4.3) U/ml] is significantly lower than III group [(35.2 +/- 4.4) U/ml] (P < 0.05).
Conclusion:
Selenoprotein that supplemented selenium doseis 300 microg Se/kg weight could significantly decrease blood glucose, increase the activities of Ca(2+) -ATPase on kidney and weaken the activities of plasma NOS in diabetic mice.
Insights
High-dose selenoprotein supplementation significantly reduced blood glucose and altered kidney calcium transport and nitric oxide synthase activity in diabetic mice, indicating a potential therapeutic role for selenoproteins in managing diabetes.
Area of Science:
- Biochemistry
- Endocrinology
- Pharmacology
Background:
- Diabetes mellitus is a metabolic disorder characterized by hyperglycemia.
- Alloxan-induced diabetes in mice is a common model for studying anti-diabetic agents.
- Selenoproteins play crucial roles in various physiological processes.
Purpose of the Study:
- To investigate the effects of selenoprotein on blood glucose control.
- To assess the impact of selenoprotein on calcium (Ca2+) transfer and the nitric oxide (NO) system in diabetic mice.
Main Methods:
- A diabetic mouse model was established using alloxan injection.
- Mice were divided into six groups, including control, diabetic, and diabetic groups treated with varying doses of selenoprotein or sodium selenite.
- Biochemical parameters such as blood glucose, Ca2+-ATPase activity, and nitric oxide synthase (NOS) activity were measured.
Main Results:
- The higher dose selenoprotein group (300 microg Se/kg) showed significantly lower blood glucose levels compared to the diabetic control group.
- Kidney Ca2+-ATPase activity was significantly increased in the high-dose selenoprotein group.
- Plasma NOS activity was significantly decreased in the high-dose selenoprotein group.
Conclusions:
- Selenoprotein supplementation at 300 microg Se/kg effectively reduced blood glucose in diabetic mice.
- This dose also enhanced kidney Ca2+-ATPase activity and attenuated plasma NOS activity.
- These findings suggest selenoprotein's potential as a therapeutic agent for diabetes management.
