Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Progressive EPR imaging with adaptive projection acquisition.

Yuanmu Deng1, Periannan Kuppusamy, Jay L Zweier

  • 1Davis Heart and Lung Research Institute, Division of Cardiovascular Medicine, Department of Internal Medicine, The Ohio State University College of Medicine, Columbus, OH 43210, USA.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|May 3, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Electronic cigarette vape decreases nitric oxide bioavailability in vascular smooth muscle cells via increased cytoglobin-mediated metabolism.

Free radical biology & medicine·2025
Same author

Tobacco cigarette smoking induces cerebrovascular dysfunction followed by oxidative neuronal injury with the onset of cognitive impairment.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism·2024
Same author

Nicotine inhalation and metabolism triggers AOX-mediated superoxide generation with oxidative lung injury.

The Journal of biological chemistry·2024
Same author

Improving combination therapies: targeting A2B-adenosine receptor to modulate metabolic tumor microenvironment and immunosuppression.

Journal of the National Cancer Institute·2023
Same author

Bacterial Pyocyanin Inducible Keratin 6A Accelerates Closure of Epithelial Defect under Conditions of Mitochondrial Dysfunction.

The Journal of investigative dermatology·2023
Same author

Trityl Quinodimethane Derivatives as Unimolecular Triple-Function Extracellular EPR Probes for Redox, pH, and Oxygen.

Analytical chemistry·2023

This study introduces a progressive electron paramagnetic resonance imaging (EPRI) technique with adaptive data acquisition. This method accelerates free radical imaging in biological systems by prioritizing significant projections and reconstructing images gradually.

Area of Science:

  • Biomedical Imaging
  • Biophysics
  • Free Radical Research

Background:

  • Continuous wave electron paramagnetic resonance imaging (EPRI) is crucial for visualizing free radicals in living biological systems.
  • Conventional EPRI reconstruction algorithms require complete projection datasets, making incomplete acquisitions unusable and limiting imaging speed.
  • The need for rapid acquisition and flexible image quality is paramount in dynamic biological studies.

Purpose of the Study:

  • To develop a 3-dimensional progressive EPRI technique for gradual image acquisition and reconstruction.
  • To implement an adaptive data acquisition strategy to optimize projection collection based on significance.
  • To enable flexible trade-offs between image quality and imaging time in EPRI.

Main Methods:

Related Experiment Videos

  • A 3D progressive EPRI technique based on inverse Radon transform was developed for gradual image acquisition.
  • An adaptive data acquisition strategy was proposed to select and order projections from most to least significant.
  • Computer simulations and experimental imaging validated the progressive imaging technique and adaptive acquisition.

    • Main Results:

    • The progressive EPRI technique allows gradual reconstruction of 3D EPR images from low to high resolution.
    • Adaptive data acquisition significantly reduces the number of required projections by 50-70% compared to regular methods.
    • Image acquisition can be terminated early without losing previously acquired data if resolution improvement plateaus.

    Conclusions:

    • Adaptive data acquisition combined with progressive image reconstruction accelerates EPRI acquisition and free radical visualization.
    • This approach offers enhanced flexibility, allowing researchers to balance imaging time with desired image resolution.
    • The developed technique is highly beneficial for real-time monitoring of free radical distribution in dynamic biological systems.