Related Experiment Video
Updated: Aug 18, 2026
Directed Differentiation of Hemogenic Endothelial Cells from Human Pluripotent Stem Cells
Published on: March 31, 2021
Morphological and phenotypical characterization of human endothelial progenitor cells in an early stage of
Lydia Bellik1, Fabrizio Ledda, Astrid Parenti
1Department of Preclinical and Clinical Pharmacology, University of Florence, Italy.
Abstract:
The exact phenotype and lineage of endothelial progenitor cells (EPCs) are still a matter of debate and different expansion protocols are used to obtain them. In this study, EPC expansion from peripheral blood mononuclear cells was analyzed within the first week of culture. Both the adherent and suspended cells, of which the latter usually discarded, were considered. We provide, for the first time, a systematic study of EPC phenotype and functional features within the first 3 days of culture. Moreover, within the 2nd day, both cellular fractions displayed a significant increase in endothelial marker expression which correlated with EPC properties.
