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An Orthotopic Murine Model of Human Prostate Cancer Metastasis
06:48

An Orthotopic Murine Model of Human Prostate Cancer Metastasis

Published on: September 19, 2013

Calcitriol inhibits growth response to Platelet-Derived Growth Factor-BB in human prostate cells

Nadja Nazarova1, Olga Golovko, Merja Bläuer

  • 1Department of Anatomy, Medical School, University of Tampere, 33014 Tampere, Finland. nadya.nazarova@uta.fi

The Journal of Steroid Biochemistry and Molecular Biology
|May 3, 2005
PubMed

Insights

Calcitriol, a form of Vitamin D, influences prostate cancer growth by regulating Platelet-Derived Growth Factor (PDGF) signaling. It down-regulates PDGFRbeta, potentially reducing prostate cell response to PDGF-BB, impacting cancer progression.

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Oncology

Background:

  • Calcitriol (Vitamin D) modulates cell growth and peptide growth factor signaling.
  • Platelet-Derived Growth Factor (PDGF) signaling is implicated in prostate cancer progression.

Purpose of the Study:

  • To investigate the role of calcitriol in regulating PDGF receptor expression and signaling in prostate cells.
  • To determine the effect of calcitriol on prostate cancer cell growth response to PDGF-BB.

Main Methods:

  • Quantitative RT-PCR to analyze mRNA levels of PDGF receptors.
  • Western blotting to assess protein expression of PDGF receptors.
  • Cell culture experiments to evaluate growth responses to PDGF-BB and Epidermal Growth Factor (EGF).

Main Results:

  • Prostate cancer cell lines (LNCaP, PC-3) showed lower PDGF receptor mRNA than primary stromal cells.
  • LNCaP cells expressed low PDGFRalpha and beta protein, correlating with resistance to PDGF-BB.
  • Calcitriol down-regulated PDGFRbeta expression and inhibited PDGF-mediated cell growth.
  • EGF partially restored LNCaP responsiveness to PDGF-BB by inducing PDGFRbeta.

Conclusions:

  • Calcitriol regulates PDGF receptor expression and signaling in prostate cells.
  • Calcitriol's down-regulation of PDGFRbeta may reduce prostate cancer cell responsiveness to PDGF-BB.
  • Targeting PDGFRbeta with calcitriol could be a strategy to inhibit prostate cancer growth.

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