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Yeast two-hybrid identification of prostatic proteins interacting with human sex hormone-binding globulin

Sharyn N Pope1, Ian R Lee

  • 1School of Biomedical Sciences, Curtin University of Technology, G.P.O. Box U1987, Perth, WA 6845, Australia.

The Journal of Steroid Biochemistry and Molecular Biology
|May 3, 2005
PubMed

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Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...