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Updated: Aug 18, 2026

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
Published on: December 29, 2015
Construction of bacterial artificial chromosome libraries for use in phylogenetic studies
Andrew G McCubbin1, Eric H Roalson
1School of Biological Sciences, Washington State University, Pullman, Washington 99164-4236, USA.
Abstract:
Bacterial artificial chromosome (BAC) libraries are emerging as valuable tools for investigating phylogenetic relationships at the level of genome structure. To date, BAC library construction has been restricted to a fairly small number of laboratories and species that represent a not insignificant, but a fairly small, fraction of diversity in the plant kingdom. This chapter is intended to contribute to rectifying this situation by providing protocols that facilitate BAC library construction in laboratories possessing basic molecular biology skills.
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