Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Calcium-dependent human erythrocyte cytoskeleton stability analysis through atomic force microscopy.

Fei Liu1, Hiroshi Mizukami, Sharada Sarnaik

  • 1Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, IN 46556, USA.

Journal of Structural Biology
|May 4, 2005
PubMed
Summary

Increased intracellular calcium in red blood cells (erythrocytes) stiffens their cytoskeleton. This calcium binding affects cell structure, potentially explaining changes seen in various diseases.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In situ Fe-doped thin carbon wires via AC high voltage arc discharge.

Scientific reports·2024
Same author

Effects of echinacoside on histio-central levels of active mass in middle cerebral artery occlusion rats.

Biomedical and environmental sciences : BES·2012
Same author

Female-to-male match predicted poor survival after living-donor liver transplantation-some issues needed to be clarified.

Transplantation·2012
Same author

X-ray repair cross-complementing group 1 (XRCC1) genetic polymorphisms and cervical cancer risk: a huge systematic review and meta-analysis.

PloS one·2012
Same author

A functional NQO1 609C>T polymorphism and risk of hepatocellular carcinoma in a Chinese population.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine·2012
Same author

7-difluoromethoxyl-5,4'-di-n-octylgenistein inhibits growth of gastric cancer cells through downregulating forkhead box M1.

World journal of gastroenterology·2012

Area of Science:

  • Biophysics
  • Cell Biology
  • Hematology

Background:

  • Erythrocytes with elevated intracellular calcium ions are observed in aging and diseases like sickle cell anemia, hypertension, and diabetes.
  • Altered erythrocyte cytoskeleton properties are linked to high intracellular calcium.
  • Calcium binding to calmodulin may weaken the spectrin-ankyrin-Band 3 linkage to the erythrocyte membrane.

Purpose of the Study:

  • To investigate the in vitro structural changes of healthy young erythrocyte cytoskeletons upon increased calcium ion binding to the cytoplasmic membrane.
  • To understand the direct impact of calcium on erythrocyte cytoskeleton structure and properties.

Main Methods:

  • Atomic force microscopy was employed to visualize erythrocyte cytoskeletons.
  • Quantitative image analysis was used to assess structural changes.

Related Experiment Videos

  • In vitro incubation of healthy young erythrocytes with excess calcium ions.

    • Main Results:

    • Extra calcium binding significantly increased the rigidity of the erythrocyte cytoskeleton.
    • Calcium incubation prevented spectrin aggregation during sample preparation.
    • The observed cytoskeleton morphology in calcium-treated cells resembled that of glutaraldehyde-fixed cells.

    Conclusions:

    • Increased intracellular calcium enhances erythrocyte cytoskeleton rigidity.
    • Calcium-induced changes in cytoskeleton structure may contribute to altered cell properties in various pathological conditions.
    • Atomic force microscopy provides a valuable tool for studying erythrocyte cytoskeleton dynamics.