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Capacitative calcium entry: sensing the calcium stores.

James W Putney1

  • 1Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health Department of Health and Human Services, Research Triangle Park, NC 27709, USA. putney@niehs.nih.gov

The Journal of Cell Biology
|May 4, 2005
PubMed
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Researchers identified stromal interaction molecule (STIM), a calcium-binding protein, as a key player in regulating calcium channels. STIM may act as the sensor for intracellular calcium store content, solving a long-standing cell biology mystery.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Calcium Signaling

Background:

  • Capacitative calcium entry (CCE) is crucial for cellular calcium homeostasis.
  • The signal linking intracellular calcium stores to plasma membrane CCE channels remains elusive.
  • Understanding this signaling pathway is vital for cell function and regulation.

Purpose of the Study:

  • To identify the molecular components responsible for sensing intracellular calcium store depletion.
  • To elucidate the mechanism of signal transduction from calcium stores to CCE channels.
  • To uncover the role of specific genes in calcium channel regulation.

Main Methods:

  • Utilized an RNA interference (RNAi)-based genetic screen in Drosophila.
  • Focused on selected Drosophila genes involved in cellular signaling pathways.

Related Experiment Videos

  • Investigated the function of identified genes in calcium channel activity.

    • Main Results:

    • Stromal interaction molecule (STIM), a calcium-binding protein, was identified as essential for CCE channel activation.
    • STIM plays a critical role in the signaling cascade initiated by calcium store depletion.
    • Evidence suggests STIM functions as the sensor for intracellular calcium store content.

    Conclusions:

    • STIM is a key regulator of capacitative calcium entry.
    • STIM likely acts as the direct sensor of calcium store status.
    • This finding resolves a fundamental question in calcium channel biology.