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Updated: Aug 10, 2026

Quantitation of Endothelial Cell Adhesiveness In Vitro
Published on: June 18, 2015
Biological effect of irradiation on adhesion molecules in human colon cancer cells in vitro
Takeshi Hasegawa1, Mikinori Sato, Masaaki Kurimoto
1Department of Gastroenterological Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan. hasetake@med.nagoya-cu.ac.jp
Background:
Carbohydrate antigens, such as sialyl Lewis(a) antigen (s-Le(a)) and sialyl Lewis(x) antigen (s-Le(x)), play an important role in cancer metastasis in vitro and in vivo. Currently, preoperative radiotherapy is used to prevent local recurrence of rectal cancer. We investigated the effects of X-ray irradiation on the carbohydrate antigens s-Le(a) and s-Le(x) in vitro.
Materials And Methods:
The cell surface expressions of s-Le(a) and s-Le(x) were determined by flow cytometric analysis at 24 hours after X-ray irradiation of 4 human cancer cell lines. s-Le(a) and s-Le(x) functions were quantitated using a monolayer cell adhesion assay with human umbilical vein endothelial cells (HUVECs).
Results:
The cell surface expressions of s-Le(a) and s-Le(x) decreased at 24 hours after irradiation. s-Le(a) adhesion to HUVECs monolayers similarly decreased at 24 hours after irradiation.
Conclusion:
These results may indicate a role for X-ray irradiation in the reduction of liver metastasis in patients with colon cancer.
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