Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tracking topoisomerase activity at the single-molecule level.

G Charvin1, T R Strick, D Bensimon

  • 1LPS, ENS, UMR 8550 CNRS, 75231 Paris Cedex 05, France. Gilles.Charvin@lps.ens.fr

Annual Review of Biophysics and Biomolecular Structure
|May 5, 2005
PubMed
Summary

New single-molecule DNA techniques reveal how topoisomerases (enzymes controlling DNA structure) function. These studies detail enzyme activity, uncovering insights into DNA topology and in vivo mechanisms.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transcription-Coupled Repair: From Cells to Single Molecules and Back Again.

Journal of molecular biology·2019
Same author

Single-molecule characterization of extrinsic transcription termination by Sen1 helicase.

Nature communications·2019
Same author

Preparation of DNA Substrates and Functionalized Glass Surfaces for Correlative Nanomanipulation and Colocalization (NanoCOSM) of Single Molecules.

Methods in enzymology·2017
Same author

Stopped in its tracks: the RNA polymerase molecular motor as a robust sensor of DNA damage.

DNA repair·2014
Same author

Validation of a clinical evaluation score for irritative dermatitis: SCOREPI.

Journal of the European Academy of Dermatology and Venereology : JEADV·2012
Same author

Topological characterization of the DnaA-oriC complex using single-molecule nanomanipuation.

Nucleic acids research·2012

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Enzymology

Background:

  • DNA topology is crucial for DNA replication and transcription.
  • Type I and II topoisomerases are essential enzymes that manage DNA topology.
  • Previous studies were limited in characterizing topoisomerase activity at a molecular level.

Purpose of the Study:

  • To investigate the mechanism of type I and II topoisomerases using novel single-molecule techniques.
  • To characterize key enzymatic activities including processivity and chiral discrimination.
  • To understand the influence of ATP concentration, DNA supercoiling, and tension on topoisomerase function.

Main Methods:

  • Utilized advanced single-molecule assays to manipulate DNA.
  • Studied the uncoiling of single supercoiled DNA molecules.

Related Experiment Videos

  • Analyzed the unlinking of intertwined DNA molecules.

    • Main Results:

    • Detailed characterization of topoisomerase activity was achieved.
    • Enzymatic rate dependence on ATP concentration, DNA supercoiling, and tension was elucidated.
    • Chiral discrimination capabilities of topoisomerases were identified.

    Conclusions:

    • Single-molecule assays provide unprecedented insights into topoisomerase mechanisms.
    • These findings enhance our understanding of topoisomerase function in vivo.
    • The study opens new avenues for exploring DNA topology and enzyme dynamics.