Related Experiment Video
Updated: Aug 18, 2026

Coupled Assays for Monitoring Protein Refolding in Saccharomyces cerevisiae
Published on: July 9, 2013
Transcriptome analysis of recombinant protein secretion by Aspergillus nidulans and the unfolded-protein response in
Andrew H Sims1, Manda E Gent, Karin Lanthaler
1Faculty of Life Sciences, The University of Manchester, Oxford Road, Manchester M13 9PT, UK.
Abstract:
Filamentous fungi have a high capacity for producing large amounts of secreted proteins, a property that has been exploited for commercial production of recombinant proteins. However, the secretory pathway, which is key to the production of extracellular proteins, is rather poorly characterized in filamentous fungi compared to yeast. We report the effects of recombinant protein secretion on gene expression levels in Aspergillus nidulans by directly comparing a bovine chymosin-producing strain with its parental wild-type strain in continuous culture by using expressed sequence tag microarrays. This approach demonstrated more subtle and specific changes in gene expression than those observed when mimicking the effects of protein overproduction by using a secretion blocker. The impact of overexpressing a secreted recombinant protein more closely resembles the unfolded-protein response in vivo.
Related Concept Videos
The Unfolded Protein Response
Regulation of the Unfolded Protein Response
Protein Folding Quality Check in the RER
Ribosome Profiling
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Leaky Scanning
Proteins: From Genes to Degradation
Transcription is the synthesis of RNA molecules by RNA...
