Related Experiment Video
Updated: Aug 18, 2026

The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective
Published on: June 16, 2022
Lactococcus lactis SpOx spontaneous mutants: a family of oxidative-stress-resistant dairy strains
Tatiana Rochat1, Jean-Jacques Gratadoux, Gérard Corthier
1Unité de Recherches Laitières et de Génetique, INRA, Domaine de Vilvert, 78352 Jouy en Josas cedex, France.
Abstract:
Numerous industrial bacteria generate hydrogen peroxide (H(2)O(2)), which may inhibit the growth of other bacteria in mixed ecosystems. We isolated spontaneous oxidative-stress-resistant (SpOx) Lactococcus lactis mutants by using a natural selection method with milk-adapted strains on dairy culture medium containing H(2)O(2). Three SpOx mutants displayed greater H(2)O(2) resistance. One of them, SpOx3, demonstrated better behavior in different oxidative-stress situations: (i) higher long-term survival upon aeration in LM17 and milk and (ii) the ability to grow with H(2)O(2)-producing Lactobacillus delbrueckii subsp. delbrueckii strains. Furthermore, the transit kinetics of the SpOx3 mutant in the digestive tract of a human flora-associated mouse model was not affected.
Related Concept Videos
Microbes in the Production of Fermented Foods
Microbes in Food Production
Clinical Significance of Antibiotic Resistance
Evolution of New Traits in Microbes
Other Stress Responses in Bacteria
