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Updated: Aug 18, 2026
Endoscopic Cholesteatoma Surgery
Published on: January 19, 2022
[Angiogenic factors in external auditory canal cholesteatoma-fibroblast cell culture]
1Universitäts-Hals-Nasen-Ohren-Klinik, Mannheim. ramin.naim@hno.ma.uni-heidelberg.de
Background:
The external auditory canal cholesteatoma (EACC) is characterized by hyperproliferation of the epithelial and subepithelial tissue. Compared to normal meatal skin, strong expression of FGF-2 and VEGF had previously been detected. Many authors reported that FGF-2 acts via VEGF and is induced by hypoxia. Hypoxia seems to be pivotal for establishing EACC. Therefore, human EACC fibroblasts were investigated by incubating with FGF-2 and determining VEGF.
Patients And Methods:
We harvested fibroblasts from human EACC and normal meatal skin and incubated the fibroblast culture with 50 ng/ml FGF-2 and determined VEGF concentrations after 1-4 days.
Results:
Compared to untreated fibroblast cultures, there was a significant increase of VEGF concentration (p<0.05). However, there was no significant difference between the proliferation quantities.
Conclusion:
VEGF and FGF-2 are possibly involved in a cascade of growth factor activities, which modulates their concentration in human-derived EACC fibroblast culture. Exogenous FGF-2 increased fibroblast expression for VEGF, which is a major autocrine mediator of FGF-2-induced angiogenesis and proliferation.
