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Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray
Published on: April 25, 2014
Isothermal RNA sequence amplification method for rapid antituberculosis drug susceptibility testing of Mycobacterium
Shunji Takakura1, Shigeo Tsuchiya, Naoko Fujihara
1Department of Clinical Laboratory Medicine, Graduate School of Medicine, Kyoto University, 54 Shogoin-Kawaharacho, Kyoto, 606-8507, Japan. stakakr@kuhp.kyoto-u.ac.jp
Journal of Clinical Microbiology
|May 6, 2005
Abstract:
RNA transcript quantification by an isothermal sequence amplification reaction was evaluated for susceptibility testing of 15 Mycobacterium tuberculosis strains. Agreement with the proportion method on Ogawa egg medium and the BACTEC MGIT 960 system was 100 and 87% for rifampin, 93 and 100% for isoniazid, 60 and 53% for ethambutol, and 80 and 80% for streptomycin, respectively.
