Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Modular expression and secretion vectors for Bacillus subtilis.

V Nagarajan1, H Albertson, M Chen

  • 1Central Research and Development Division, E.I. duPont de Nemours Company, Wilmington, DE 19880-0228.

Gene
|May 1, 1992
PubMed
Summary

A new modular vector system enables efficient extracellular protein production in Bacillus subtilis. This system utilizes four secretion vectors and reporter proteins for optimized cloning and genetic manipulation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Procedures for the analysis and purification of his-tagged proteins.

Methods in molecular medicine·2011
Same author

Detection and Immobilization of Proteins Containing the 6xHis Tag.

Methods in molecular medicine·2011
Same author

Oncogenic c-Ki-ras but not oncogenic c-Ha-ras up-regulates CEA expression and disrupts basolateral polarity in colon epithelial cells.

The Journal of biological chemistry·1997
Same author

Transient gene expression from yeast artificial chromosome DNA in mammalian cells is enhanced by adenovirus.

Nucleic acids research·1997
Same author

Wiskott-Aldrich syndrome protein is associated with the adapter protein Grb2 and the epidermal growth factor receptor in living cells.

Molecular biology of the cell·1997
Same author

Role of ribosomes in reinitiation of membrane insertion of internal transmembrane segments in a polytopic membrane protein.

Biochemistry·1997

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biotechnology

Background:

  • Extracellular protein production in Bacillus subtilis is crucial for industrial applications.
  • Developing efficient secretion systems is essential for maximizing protein yields.

Purpose of the Study:

  • To develop a modular vector system for enhanced extracellular protein production in Bacillus subtilis.
  • To facilitate cloning, genetic manipulation, and media optimization for protein secretion.

Main Methods:

  • Developed four secretion vectors based on Bacillus amyloliquefaciens protease, barnase, and levansucrase genes.
  • Incorporated compatible restriction sites for seamless gene insertion.
  • Fused reporter proteins (Protein A, levansucrase, alkaline phosphatase) to signal peptides.

Related Experiment Videos

Main Results:

  • The modular vector system demonstrated functionality in both Bacillus subtilis and Escherichia coli.
  • Reporter protein fusions enabled efficient cloning and genetic manipulation.
  • The system allows for comparative studies of protein secretion mechanisms.

Conclusions:

  • The developed modular vector system provides a versatile platform for heterologous protein secretion in Bacillus subtilis.
  • This system simplifies protein production optimization through reporter protein fusions.
  • The ability to function in E. coli facilitates comparative secretion studies.