Related Experiment Video
Updated: Aug 18, 2026
Collection, Isolation, and Flow Cytometric Analysis of Human Endocervical Samples
Published on: July 6, 2014
Transepithelial sampling of the uterine ectocervix with a stiff-bristled, spiral-shaped brush
Devansu Tewari1, Neal M Lonky, Sharon P Wilczynski
1Department of Obstetrics and Gynecology, Division of Gynecologic Oncology, University of California Irvine, Irvine, CA, USA.
Objective:
To measure the depth of sampling of the uterine ectocervix with the use of a stiff-bristled, spiral-shaped brush (SpiraBrush Cx; Trylon Corporation, Torrance, CA).
Materials And Methods:
Eligible hysterectomy specimens were identified. The ectocervix was brushed with an inked SpiraBrush Cx in four quadrants with either light (gentle) or heavy (forceful) pressure. The depth of sampling was determined histologically.
Results:
Four normal uteri were sampled a total of 15 times. The entire epithelium was removed to the epithelial-stromal junction in three specimens and in one sample only a few basal cells remained with light pressure. The depth of sampling ranged from 0.2 mm to 0.4 mm. Heavy pressure resulted in the entire removal of epithelium in six samples, with the depth of sampling ranging from 0.2 mm to 0.7 mm. The stroma was disrupted in one sample. Results were uninformative in five samples.
Conclusions:
Sampling of the uterine ectocervix with the SpiraBrush Cx seems to be transepithelial in most cases.
