Effect of iontophoresis on skin permeation of defibrase
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Published on: September 13, 2022
Hui-ying Zhao1, Qi-peng Yuan, Jun-min Zheng
1School of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China. hyzhao@mail.buct.edu.cn
Aim:
To investigate the effect of iontophoresis on skin permeation of defibrase.
Methods:
Iontophoresis was carried out in side-by-side chambers, excised rat skin membrane (RSM) or human epidermis membrane (HEM). The effects of electrode polarity, permeation medium pH and ionic strength were evaluated.
Results:
Permeation of defibrase caused by anodal iontophoresis was more effective [the apparent permeability coefficient was (1.2 +/- 0.4) x 10(-4) cm x h(-1)] than that of cathodal iontophoresis [(4.3 +/- 1.4) x 10(-5) cm x h(-1)]. The amount of permeated defibrase caused by anodal iontophoresis in pH 7.4 medium was (25 +/- 5) x 10(-14) mol x cm(-2), which was higher than that of in pH 6. 4 permeation medium [(15 +/- 4) x 10(-14) mol x cm(-2)].
Conclusion:
Iontophoresis could enhance skin permeation of defibrase. Electroosmotic flow effect played an important role.
