Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sequence-dependent DNA immobilization: specific versus nonspecific contributions.

Lauren K Wolf1, Yang Gao, Rosina M Georgiadis

  • 1Department of Chemistry, Boston University, Boston, Massachusetts 02215, USA.

Langmuir : the ACS Journal of Surfaces and Colloids
|May 7, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cutting the cord: virtual machines for real instrumental analysis not just at the instrument.

Analytical and bioanalytical chemistry·2018
Same author

[Effect of perioperative treatment with ambroxol on lung cancer patients after video-assisted thoracic surgery lobectomy].

Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences·2014
Same author

Overexpression of FABP7 promotes cell growth and predicts poor prognosis of clear cell renal cell carcinoma.

Urologic oncology·2014
Same author

Preparation and pharmacokinetics study on gastro-floating sustained-release tablets of troxipide.

Drug development and industrial pharmacy·2014
Same author

De novo design of an endohedral heteronuclear dimetallofullerene (U-Gd)@C60 with exceptional structural and electronic properties.

Chemphyschem : a European journal of chemical physics and physical chemistry·2014
Same author

Accelerating Multiagent Reinforcement Learning by Equilibrium Transfer.

IEEE transactions on cybernetics·2014
Same journal

Metal-Organic Framework Multizyme Colloids with Joint Antioxidant and Protease Function.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Morphology Engineering of Co<sub>3</sub>O<sub>4</sub> via Cetyltrimethylammonium Bromide-Mediated ZIF-67 Synthesis for Efficient Photo-Assisted Electrooxidation of Methanol.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Speciation of Silanol Groups on Commercial Precipitated Silicas via IR Spectroscopy.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Regenerable PVA Hydrogel-Functionalized Optical Fiber Sensor for Ultra-Trace Detection of Berberine Hydrochloride.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Hydrogen Plasma-Driven Surface Defect Engineering of ZnO Nanorods: Correlating Electronic Structure and Photoelectrochemical Performance.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Cooperative Self-Assembly of Nanoparticle-Encapsulating Hybrid Protein Cages.

Langmuir : the ACS journal of surfaces and colloids·2026
See all related articles

Oligonucleotide immobilization on gold surfaces is influenced by DNA sequence. Specific and nonspecific interactions dictate adsorption kinetics and film coverage, impacting DNA-thiol attachment mechanisms.

Area of Science:

  • Surface science
  • Biotechnology
  • Materials science

Background:

  • DNA immobilization on gold surfaces is crucial for biosensors and nanotechnology.
  • Understanding the kinetics and mechanisms of DNA attachment is essential for optimizing surface functionalization.

Purpose of the Study:

  • To investigate the sequence-dependent kinetics of single-strand oligonucleotide immobilization on gold surfaces.
  • To differentiate the roles of specific thiol-gold interactions and nonspecific DNA-surface interactions in DNA attachment.

Main Methods:

  • Systematic study of in situ sequence-dependent kinetics.
  • Measurement of film coverage for thiolated and nonthiolated homo-oligonucleotides over time.
  • Analysis of adsorption and rinsing behavior for different DNA sequences.

Related Experiment Videos

Main Results:

  • Sequence-dependent nonspecific interactions significantly influence DNA-thiol immobilization kinetics and film coverage.
  • Strong initial nonspecific binding (e.g., poly(dA)-thiol) hinders subsequent thiol adsorption, reducing final coverage.
  • Weak initial nonspecific binding (e.g., poly(dT)-thiol) allows higher film coverage and follows Langmuir kinetics.
  • Thiolated strands adopt an upright orientation with base-stacking, unlike nonthiolated strands.

Conclusions:

  • Both specific thiol-gold and nonspecific DNA-surface interactions are critical in DNA-thiol film formation.
  • Oligonucleotide sequence composition dictates the interplay of these forces, affecting adsorption rate and equilibrium coverage.
  • Controlling sequence-dependent nonspecific interactions is key to optimizing DNA-thiol surface functionalization.