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Patterning poly(organophosphazenes) for selective cell adhesion applications.

Eric W Barrett1, Mwita V B Phelps, Ricardo J Silva

  • 1Department of Chemistry, 104 Chemistry Building, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.

Biomacromolecules
|May 10, 2005
PubMed
Summary

Researchers developed patterned polyphosphazene materials to control neuroblastoma cell adhesion. Certain polymers (-CAPs) prevented cell attachment, while others (+CAPs) promoted it, enabling selective cell culturing.

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Area of Science:

  • Materials Science
  • Biomedical Engineering
  • Polymer Chemistry

Background:

  • Developing selective cell culture substrates is crucial for tissue engineering and neuroscience research.
  • Polyphosphazenes offer tunable properties for biomaterial applications.
  • Controlling cellular adhesion at the microscale is challenging but essential for patterned cell growth.

Purpose of the Study:

  • To synthesize and screen polyphosphazenes with varying hydrophilicities for differential cellular adhesion.
  • To identify specific polyphosphazene materials that either promote or prevent adhesion of SK-N-BE(2c) human neuroblastoma cells.
  • To demonstrate the ability to create micropatterned surfaces for selective neuroblastoma cell culture.

Main Methods:

  • Five distinct polyphosphazenes were synthesized, varying in hydrophilic properties.

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  • Polymer films were created and tested in vitro for cellular adhesion using the SK-N-BE(2c) cell line.
  • Negative cellular adhesive properties (-CAPs) were observed with poly[bis(trifluoroethoxy)phosphazene] (TFE) and poly[bis(methoxyethoxyethoxy)phosphazene] (MEEP).
  • Positive cellular adhesive properties (+CAPs) were observed with poly[(methoxyethoxyethoxy)(1.0)(carboxylatophenoxy)(1.0)phosphazene] (PMCPP), poly[(methoxyethoxyethoxy)(1.0)(cinnamyloxy)(1.0)phosphazene] (PMCP), and poly[(methoxyethoxyethoxy)(1.0)(p-methylphenoxy)(1.0)phosphazene] (PMMP).
  • Micropatterned films of -CAP and +CAP polymers were co-patterned onto glass substrates.
  • Neuroblastoma cells were cultured on these micropatterned films for one week.

    • Main Results:

    • Neuroblastoma cells selectively adhered to the +CAP microfeatures, with over 60% selectivity observed.
    • TFE demonstrated utility as both a negative cellular adhesive (-CAP) material and an insulating layer.
    • PMCP served as a conductive positive cellular adhesive (+CAP) layer, showcasing multifunctional material capabilities.

    Conclusions:

    • Polyphosphazenes can be designed to exhibit distinct positive (+CAP) or negative (-CAP) cellular adhesive properties.
    • Micropatterned surfaces created with these polyphosphazenes enable highly selective culture of SK-N-BE(2c) neuroblastoma cells.
    • Single polyphosphazene materials can possess multiple functionalities, such as adhesion control and conductivity, for advanced applications.