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Updated: Aug 18, 2026
Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System
Published on: December 7, 2012
White blood cell fragments in platelet concentrates prepared by the platelet-rich plasma or buffy-coat methods
M J Dijkstra-Tiekstra1, C E van der Schoot, R N I Pietersz
1Sanquin Blood Bank North-west Region, 100 AC Amsterdam, the Netherlands.
Background And Objectives:
White blood cell (WBC) fragments in platelet concentrates (PCs) may induce allo-immunization in the recipient.
Materials And Methods:
As the level of WBC fragments can differ between PCs produced using different methods, we compared PCs prepared by using the buffy-coat method (BC-PCs) in plasma or platelet additive solution (Composol) and PCs prepared using the platelet-rich plasma method (PRP-PCs).
Results:
Post-filtration results revealed identical levels of WBC, but significantly higher CD62p expression and a significantly lower amount of total DNA, cell-free DNA and number of WBC fragments (0.6 vs. 4.2 WBC equivalents/microl) in PRP-PCs than in BC-PCs in either plasma or Composol.
Conclusions:
The number of WBC fragments is significantly higher in BC-PCs than in PRP-PCs.
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