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Platelet-derived microparticles induce angiogenesis and stimulate post-ischemic revascularization

Alexander Brill1, Olga Dashevsky, Julia Rivo

  • 1Coagulation Unit, Hematology Department, Hadassah Hebrew University Medical Center, Jerusalem 91120, Israel. brilla@hadassah.org.il

Cardiovascular Research
|May 10, 2005
PubMed
Abstract

Insights

Platelet-derived microparticles (PMP) promote new blood vessel growth (angiogenesis) in laboratory models and in animals with heart ischemia. PMP may improve revascularization in ischemic conditions.

Area of Science:

  • Cardiovascular Biology
  • Cell Biology
  • Biomedical Research

Background:

  • Platelet activation releases microparticles, but their role in angiogenesis is unclear.
  • Platelet-derived microparticles (PMP) are implicated in various physiological and pathological processes.

Purpose of the Study:

  • To investigate the effect of PMP on angiogenesis.
  • To elucidate the underlying mechanisms of PMP-mediated angiogenesis.

Main Methods:

  • In vitro studies: rat aortic ring assay, endothelial cell invasion assays.
  • In vivo studies: agarose bead transplantation in mice, artificial cardiac ischemia model in rats.

Main Results:

  • PMP demonstrated a dose-dependent pro-angiogenic effect in vitro, mediated by VEGF, PI 3-kinase, Src kinase, and ERK.
  • PMP enhanced endothelial cell invasion, involving VEGF, heparanase, and PDGF.
  • PMP promoted angiogenesis in vivo and improved capillary function in a rat model of chronic myocardial ischemia.

Conclusions:

  • PMP significantly induce angiogenesis both in vitro and in vivo.
  • PMP administration may enhance revascularization in ischemic myocardium, suggesting therapeutic potential.

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