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Updated: Aug 18, 2026

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery
Published on: April 17, 2012
Chitosan beads as molecularly imprinted polymer matrix for selective separation of proteins
Tian-Ying Guo1, Yong-Qing Xia, Jin Wang
1State Key Laboratory of Functional Polymer Materials for Adsorption and Separation, Institute of Polymer Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China. tyguo@nankai.edu.cn
Abstract:
Two kinds of molecularly imprinted polymers were prepared using hemoglobin as the imprinting molecule, acrylamide as the functional monomer, chitosan beads and maleic anhydride-modified chitosan beads as matrixes, respectively. Static adsorbing experimental results showed that an equal class of adsorption was formed in the imprinted polymers and the adsorption equilibrium constant and the maximum adsorption capacity were evaluated. Chromatographic characteristics showed that the column bedded with the hemoglobin imprinted beads could separate hemoglobin and bovine serum albumin effectively from their mixture, which indicates that the imprinted beads have very higher selectivity for hemoglobin than the non-imprinted with the same chemical composition.
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