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Main phase transitions in supported lipid single-bilayer.

A Charrier1, F Thibaudau

  • 1Centre de Recherche en Matière Condensée et Nanosciences, Centre National de la Recherche Scientifique, Université de la Méditerranée, Marseille, France. charrier@crmcn.univ-mrs.fr

Biophysical Journal
|May 10, 2005
PubMed
Summary

This study reveals two distinct gel-to-fluid phase transitions in supported lipid bilayers, showing each leaflet melts independently. These transitions are explained by a thermodynamic model incorporating surface tension changes.

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Area of Science:

  • Materials Science
  • Biophysics
  • Physical Chemistry

Background:

  • Phospholipidic bilayers are fundamental to cell membranes.
  • Understanding lipid bilayer phase transitions is crucial for membrane biophysics.
  • Supported lipid bilayers provide model systems for studying membrane behavior.

Purpose of the Study:

  • To investigate the phase transitions of a single phospholipidic bilayer supported on mica.
  • To characterize the gel (L(beta)) to fluid (L(alpha)) transitions.
  • To interpret the observed transitions using thermodynamic principles.

Main Methods:

  • Real-time temperature-controlled atomic force microscopy (AFM) was employed.
  • The study focused on a single phospholipidic bilayer system.

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  • Mica served as the substrate for the supported bilayer.

    • Main Results:

    • Two distinct phase transitions were observed in the lipid bilayer.
    • These transitions correspond to the independent melting of each leaflet.
    • The ratio of phases and transition broadening were analyzed.

    Conclusions:

    • The findings support the independent melting of each leaflet in a supported bilayer.
    • A thermodynamic framework with variable surface tension explains the observed transitions.
    • Experimental data align with the proposed thermodynamic model.