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Related Experiment Videos

Novel CD47-dependent intercellular adhesion modulates cell migration.

Robert A Rebres1, Kimberly Kajihara, Eric J Brown

  • 1Program in Microbial Pathogenesis and Host Defense, University of California, San Francisco, California, USA.

Journal of Cellular Physiology
|May 10, 2005
PubMed
Summary

CD47 protein on cell surfaces promotes cell-cell adhesion and aggregation, even without known binding partners. This CD47-mediated adhesion influences cell migration, impacting barrier function and chemoattractant-driven movement.

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Area of Science:

  • Cell Biology
  • Immunology
  • Biochemistry

Background:

  • CD47, also known as Integrin Associated Protein, is a plasma membrane protein involved in cell adhesion.
  • It interacts with ligands like thrombospondin (TSP) and SIRPalpha1, and modulates integrin binding avidity.

Purpose of the Study:

  • To investigate the role of CD47 in intercellular adhesion and cell aggregation independent of its known ligands.
  • To explore CD47's influence on cell migration and barrier function.

Main Methods:

  • Fibroblast and Jurkat lymphocyte cell culture.
  • Assessment of cell-cell adhesion and aggregation.
  • Analysis of GTP-bound rac levels.
  • Migration assays through fibroblast monolayers.
  • Inhibition studies using pertussis toxin, C. difficile toxin B, and CD47-binding peptides.

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Main Results:

  • Fibroblast CD47 expression induces cell aggregation without active integrins, SIRPalpha1, or TSP.
  • CD47-expressing cells preferentially adhere to each other, requiring serum or TSP peptide stimulation.
  • CD47 expression on fibroblasts increases barrier function, reducing spontaneous Jurkat lymphocyte migration.
  • Lymphocyte migration through fibroblast monolayers is dependent on CD47 expression on both cell types.

Conclusions:

  • CD47 can mediate intercellular adhesion and aggregation independently of its known ligands.
  • CD47 signaling, involving heterotrimeric and rho family GTPases, plays a role in cell-cell interactions.
  • CD47 expression modulates cell migration and barrier function, with implications for cell-cell communication.