Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ultra-high sensitivity multi-photon detection imaging in proteomics analyses.

Oliver Kleiner1, David A Price, Natasha Ossetrova

  • 1Department of Medicine, Centre for Molecular Medicine, University College London, London, UK.

Proteomics
|May 10, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biochemical Basis for LRP1 Interaction With Rift Valley Fever Virus Glycoprotein and Its Role in Viral Entry.

Journal of molecular biology·2026
Same author

Robust HLA-B-restricted CD8+ T-cell responses in chronic HBV infection.

JHEP reports : innovation in hepatology·2026
Same author

Activation of the Lectin Pathway Drives Persistent Complement Dysregulation in Long COVID.

Immunology·2026
Same author

CXCR5 identifies stem-like resident memory CD8⁺ T cells enriched for latent EBV specificity in tonsils.

Science advances·2026
Same author

Machine Learning Analysis of Post-Acute COVID Symptoms Identifies Distinct Clusters, Severity Groups, and Trajectories.

medRxiv : the preprint server for health sciences·2025
Same author

LRP1 facilitates Jamestown Canyon virus infection of neurons.

Journal of virology·2025

New multicolor, multi-photon detection (MPD) methods enhance radioactive labeling detection for functional proteomics. This technique achieves high sensitivity and quantitative accuracy for zeptomole to attomole protein levels.

Area of Science:

  • Proteomics
  • Biochemistry
  • Analytical Chemistry

Background:

  • Two-dimensional gel electrophoresis (2D-PAGE) is a key technique in proteomics.
  • Sensitive detection of low-abundance proteins is crucial for functional proteomics.
  • Current methods for detecting radiolabeled proteins have limitations in sensitivity and quantification.

Purpose of the Study:

  • To introduce and validate new multicolor, multi-photon detection (MPD) methods for routine and quantitative detection of radiolabeled proteins on 2D gels.
  • To demonstrate the advantages of MPD for functional proteomics, including enhanced sensitivity and multiplexing capabilities.

Main Methods:

  • Utilized iodine-125 (125I) and iodine-131 (131I) radioisotopes for protein labeling.
  • Employed multicolor, multi-photon detection (MPD) technology with single particle detectors.

Related Experiment Videos

  • Applied coincident detection of particles/photons for enhanced sensitivity with specific radionuclides.

    • Main Results:

    • Achieved routine and quantitative detection of protein spots in the zeptomole to attomole range.
    • Demonstrated a dramatic improvement in sensitivity for radiolabel detection using single particle detectors.
    • Showcased the linearity of MPD technology over six to seven orders of magnitude (60 zeptomoles to 60 femtomoles).
    • Successfully detected radionuclides below background radiation levels using coincident detection.

    Conclusions:

    • MPD methodology offers significant advantages for functional proteomics due to its high sensitivity, quantitative accuracy, and multiplexing potential.
    • The developed methods enable the monitoring of acidic/phosphorylated proteins in small biological samples (e.g., 60 ng of HeLa cell proteins).
    • MPD represents a powerful advancement in the field of quantitative proteomics and biomarker discovery.