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A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro
Published on: June 16, 2022
Substance P upregulates osteoclastogenesis by activating nuclear factor kappa B in osteoclast precursors
1Department of Otolaryngology, Catholic University of Daegu School of Medicine, Daegu, South Korea. sjsohn@cu.ac.kr
Conclusion:
SP upregulates osteoclastogenesis by activating NF-kappaB in osteoclast precursors.
Objective:
Osteoclastic bone resorption is a common finding of otitis media with or without cholesteatoma. Factors upregulating osteoclastogenesis are implicated in bone resorption. The aim of this study was to investigate the effect of substance P (SP) on mouse osteoclastogenesis in vitro.
Material And Methods:
The SP dose response was analyzed in a mouse osteoclast culture induced by macrophage colony-stimulating factor and receptor activator for nuclear factor kappa B (NF-kappaB) ligand. Western blot analysis was performed to elucidate the subcellular target of SP in osteoclastogenesis.
Results:
Treatment with SP significantly increased osteoclastogenesis compared to control. SP activated nuclear translocation of NF-kappaB, which is an essential event in osteoclastogenesis in osteoclast precursors (bone marrow macrophages).
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