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Published on: August 14, 2019
An HPLC-PAD method to analyse flavonoid glycosides and styrylpyrones from Cryptocarya species (Lauraceae)
Cláudia J Nehme1, Wagner L Bastos, Adelson J de Araújo
1Núcleo de Bioensaios, Bissíntese e Ecofisiologia de Produtos Naturais, Instituto de Química, UNESP, Araraquara-SP 14800-900, Brazil.
Abstract:
An HPLC-PAD method has been developed in order to evaluate simultaneously the main secondary metabolites, flavonoid glycosides and styrylpyrones, of leaves of Cryptocarya moschata. The sample preparation, consisting of extraction, liquid-liquid extraction and centrifugation, requires minimum sample manipulation but produces high yields with reproducibility, selectivity and simplicity. HPLC on a C18 column presents each class of metabolites grouped and with good resolution of the main compounds. The experimental conditions can be used to study inter- and intra-specific variability of secondary metabolites in Cryptocarya spp.
