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Updated: Aug 18, 2026
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
Published on: November 15, 2017
[Nano flow multidimensional liquid chromatography for proteome analysis of rat liver]
Yan Wang1, Mingxia Gao, Xue Gu
1Department of Chemistry, Fudan University, Shanghai 200032, China.
Abstract:
A nano flow comprehensive multidimensional liquid chromatography was developed. By using this separation platform, complex samples can be injected, desalted, separated and analyzed in complete automatization. Coupling of the strong cation-exchange (SCX) with capillary reversed-phase liquid chromatography (cRPLC) is described for proteomics of rat liver. A step gradient was applied for SCX column, and a linear gradient was applied for RPLC. The flow rate through the nano scale analytical column during the separation process was maintained at 200 nL/min. The peak capacity for the nano-2D-LC was up to 620 under these conditions.
