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A Parasite Rescue and Transformation Assay for Antileishmanial Screening Against Intracellular Leishmania donovani Amastigotes in THP1 Human Acute Monocytic Leukemia Cell Line
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Published on: December 30, 2012

Leishmanization: use of an old method for evaluation of candidate vaccines against leishmaniasis

Ali Khamesipour1, Yahya Dowlati, Ali Asilian

  • 1Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, P.O. Box 14155-6383, Tehran 14166, Iran. khamesipour_ali@yahoo.com

Vaccine
|May 11, 2005
PubMed

To establish the safety and reproducibility of live challenge with Leishmania major, a single frozen stabilate was used in two open, single-arm leishmanization (LZ) trials. A total of 42 inoculations in 28 male adult volunteers were followed until complete healing. Lesions induced by LZ are as diverse as natural infection, but much milder. Total protection was seen in 100% (11/11) of recovered volunteers. LZ used as live challenge to test vaccine candidates reduces the required sample size, is cost effective for surrogate markers studies and will induce protection in the participants who are not protected by candidate vaccines.

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